Linkage mapping, molecular cloning and functional analysis of soybean gene Fg3 encoding flavonol 3-O-glucoside/galactoside (1 → 2) glucosyltransferase
Identifieur interne : 000563 ( Main/Exploration ); précédent : 000562; suivant : 000564Linkage mapping, molecular cloning and functional analysis of soybean gene Fg3 encoding flavonol 3-O-glucoside/galactoside (1 → 2) glucosyltransferase
Auteurs : Shaokang Di ; Fan Yan ; Felipe Rojas Rodas [Colombie] ; Tito O. Rodriguez ; Yoshinori Murai ; Tsukasa Iwashina ; Satoko Sugawara ; Tetsuya Mori ; Ryo Nakabayashi ; Keiko Yonekura-Sakakibara ; Kazuki Saito ; Ryoji TakahashiSource :
- BMC Plant Biology [ 1471-2229 ] ; 2015.
Abstract
Flavonol glycosides (FGs) are major components of soybean leaves and there are substantial differences in FG composition among genotypes. The first objective of this study was to identify genes responsible for FG biosynthesis and to locate them in the soybean genome. The second objective was to clone the candidate genes and to verify their function. Recombinant inbred lines (RILs) were developed from a cross between cultivars Nezumisaya and Harosoy.
HPLC comparison with authentic samples suggested that FGs having glucose at the 2″-position of glucose or galactose that is bound to the 3-position of kaempferol were present in Nezumisaya, whereas FGs of Harosoy were devoid of 2″-glucose. Conversely, FGs having glucose at the 6″-position of glucose or galactose that is bound to the 3-position of kaempferol were present in Harosoy, whereas these FGs were absent in Nezumisaya. Genetic analysis suggested that two genes control the pattern of attachment of these sugar moieties in FGs. One of the genes may be responsible for attachment of glucose to the 2″-position, probably encoding for a flavonol 3-
The online version of this article (doi:10.1186/s12870-015-0504-7) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s12870-015-0504-7
PubMed: 26002063
PubMed Central: 4494776
Affiliations:
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Rodriguez</name><affiliation><nlm:aff id="Aff1">Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8518 Japan</nlm:aff><wicri:noCountry code="subfield">Ibaraki 305-8518 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Murai, Yoshinori" sort="Murai, Yoshinori" uniqKey="Murai Y" first="Yoshinori" last="Murai">Yoshinori Murai</name><affiliation><nlm:aff id="Aff2">Department of Botany, National Museum of Nature and Science, Tsukuba, Ibaraki 305-0005 Japan</nlm:aff><wicri:noCountry code="subfield">Ibaraki 305-0005 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Iwashina, Tsukasa" sort="Iwashina, Tsukasa" uniqKey="Iwashina T" first="Tsukasa" last="Iwashina">Tsukasa Iwashina</name><affiliation><nlm:aff id="Aff2">Department of Botany, National Museum of Nature and Science, Tsukuba, Ibaraki 305-0005 Japan</nlm:aff><wicri:noCountry code="subfield">Ibaraki 305-0005 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Sugawara, Satoko" sort="Sugawara, Satoko" uniqKey="Sugawara S" first="Satoko" last="Sugawara">Satoko Sugawara</name><affiliation><nlm:aff id="Aff3">RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045 Japan</nlm:aff><wicri:noCountry code="subfield">Kanagawa 230-0045 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Mori, Tetsuya" sort="Mori, Tetsuya" uniqKey="Mori T" first="Tetsuya" last="Mori">Tetsuya Mori</name><affiliation><nlm:aff id="Aff3">RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045 Japan</nlm:aff><wicri:noCountry code="subfield">Kanagawa 230-0045 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Nakabayashi, Ryo" sort="Nakabayashi, Ryo" uniqKey="Nakabayashi R" first="Ryo" last="Nakabayashi">Ryo Nakabayashi</name><affiliation><nlm:aff id="Aff3">RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045 Japan</nlm:aff><wicri:noCountry code="subfield">Kanagawa 230-0045 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Yonekura Sakakibara, Keiko" sort="Yonekura Sakakibara, Keiko" uniqKey="Yonekura Sakakibara K" first="Keiko" last="Yonekura-Sakakibara">Keiko Yonekura-Sakakibara</name><affiliation><nlm:aff id="Aff3">RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045 Japan</nlm:aff><wicri:noCountry code="subfield">Kanagawa 230-0045 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Saito, Kazuki" sort="Saito, Kazuki" uniqKey="Saito K" first="Kazuki" last="Saito">Kazuki Saito</name><affiliation><nlm:aff id="Aff3">RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045 Japan</nlm:aff><wicri:noCountry code="subfield">Kanagawa 230-0045 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Takahashi, Ryoji" sort="Takahashi, Ryoji" uniqKey="Takahashi R" first="Ryoji" last="Takahashi">Ryoji Takahashi</name><affiliation><nlm:aff id="Aff1">Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8518 Japan</nlm:aff><wicri:noCountry code="subfield">Ibaraki 305-8518 Japan</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff4">National Institute of Crop Science, Tsukuba, Ibaraki 305-8518 Japan</nlm:aff><wicri:noCountry code="subfield">Ibaraki 305-8518 Japan</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26002063</idno><idno type="pmc">4494776</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494776</idno><idno type="RBID">PMC:4494776</idno><idno type="doi">10.1186/s12870-015-0504-7</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000628</idno><idno type="wicri:Area/Pmc/Curation">000627</idno><idno type="wicri:Area/Pmc/Checkpoint">000443</idno><idno type="wicri:Area/Ncbi/Merge">001B46</idno><idno type="wicri:Area/Ncbi/Curation">001B46</idno><idno type="wicri:Area/Ncbi/Checkpoint">001B46</idno><idno type="wicri:Area/Main/Merge">000563</idno><idno type="wicri:Area/Main/Curation">000563</idno><idno type="wicri:Area/Main/Exploration">000563</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Linkage mapping, molecular cloning and functional analysis of soybean gene <italic>Fg3</italic> encoding flavonol 3-<italic>O</italic>-glucoside/galactoside (1 → 2) glucosyltransferase</title><author><name sortKey="Di, Shaokang" sort="Di, Shaokang" uniqKey="Di S" first="Shaokang" last="Di">Shaokang Di</name><affiliation><nlm:aff id="Aff1">Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8518 Japan</nlm:aff><wicri:noCountry code="subfield">Ibaraki 305-8518 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Yan, Fan" sort="Yan, Fan" uniqKey="Yan F" first="Fan" last="Yan">Fan Yan</name><affiliation><nlm:aff id="Aff1">Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8518 Japan</nlm:aff><wicri:noCountry code="subfield">Ibaraki 305-8518 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Rodas, Felipe Rojas" sort="Rodas, Felipe Rojas" uniqKey="Rodas F" first="Felipe Rojas" last="Rodas">Felipe Rojas Rodas</name><affiliation><nlm:aff id="Aff1">Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8518 Japan</nlm:aff><wicri:noCountry code="subfield">Ibaraki 305-8518 Japan</wicri:noCountry></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff5">Current address: Universidad Católica de Oriente, Rionegro-Antioquia, Colombia</nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Current address: Universidad Católica de Oriente, Rionegro-Antioquia</wicri:regionArea><wicri:noRegion>Rionegro-Antioquia</wicri:noRegion></affiliation></author><author><name sortKey="Rodriguez, Tito O" sort="Rodriguez, Tito O" uniqKey="Rodriguez T" first="Tito O" last="Rodriguez">Tito O. Rodriguez</name><affiliation><nlm:aff id="Aff1">Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8518 Japan</nlm:aff><wicri:noCountry code="subfield">Ibaraki 305-8518 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Murai, Yoshinori" sort="Murai, Yoshinori" uniqKey="Murai Y" first="Yoshinori" last="Murai">Yoshinori Murai</name><affiliation><nlm:aff id="Aff2">Department of Botany, National Museum of Nature and Science, Tsukuba, Ibaraki 305-0005 Japan</nlm:aff><wicri:noCountry code="subfield">Ibaraki 305-0005 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Iwashina, Tsukasa" sort="Iwashina, Tsukasa" uniqKey="Iwashina T" first="Tsukasa" last="Iwashina">Tsukasa Iwashina</name><affiliation><nlm:aff id="Aff2">Department of Botany, National Museum of Nature and Science, Tsukuba, Ibaraki 305-0005 Japan</nlm:aff><wicri:noCountry code="subfield">Ibaraki 305-0005 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Sugawara, Satoko" sort="Sugawara, Satoko" uniqKey="Sugawara S" first="Satoko" last="Sugawara">Satoko Sugawara</name><affiliation><nlm:aff id="Aff3">RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045 Japan</nlm:aff><wicri:noCountry code="subfield">Kanagawa 230-0045 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Mori, Tetsuya" sort="Mori, Tetsuya" uniqKey="Mori T" first="Tetsuya" last="Mori">Tetsuya Mori</name><affiliation><nlm:aff id="Aff3">RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045 Japan</nlm:aff><wicri:noCountry code="subfield">Kanagawa 230-0045 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Nakabayashi, Ryo" sort="Nakabayashi, Ryo" uniqKey="Nakabayashi R" first="Ryo" last="Nakabayashi">Ryo Nakabayashi</name><affiliation><nlm:aff id="Aff3">RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045 Japan</nlm:aff><wicri:noCountry code="subfield">Kanagawa 230-0045 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Yonekura Sakakibara, Keiko" sort="Yonekura Sakakibara, Keiko" uniqKey="Yonekura Sakakibara K" first="Keiko" last="Yonekura-Sakakibara">Keiko Yonekura-Sakakibara</name><affiliation><nlm:aff id="Aff3">RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045 Japan</nlm:aff><wicri:noCountry code="subfield">Kanagawa 230-0045 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Saito, Kazuki" sort="Saito, Kazuki" uniqKey="Saito K" first="Kazuki" last="Saito">Kazuki Saito</name><affiliation><nlm:aff id="Aff3">RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045 Japan</nlm:aff><wicri:noCountry code="subfield">Kanagawa 230-0045 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Takahashi, Ryoji" sort="Takahashi, Ryoji" uniqKey="Takahashi R" first="Ryoji" last="Takahashi">Ryoji Takahashi</name><affiliation><nlm:aff id="Aff1">Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8518 Japan</nlm:aff><wicri:noCountry code="subfield">Ibaraki 305-8518 Japan</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff4">National Institute of Crop Science, Tsukuba, Ibaraki 305-8518 Japan</nlm:aff><wicri:noCountry code="subfield">Ibaraki 305-8518 Japan</wicri:noCountry></affiliation></author></analytic><series><title level="j">BMC Plant Biology</title><idno type="eISSN">1471-2229</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Flavonol glycosides (FGs) are major components of soybean leaves and there are substantial differences in FG composition among genotypes. The first objective of this study was to identify genes responsible for FG biosynthesis and to locate them in the soybean genome. The second objective was to clone the candidate genes and to verify their function. Recombinant inbred lines (RILs) were developed from a cross between cultivars Nezumisaya and Harosoy.</p></sec><sec><title>Results</title><p>HPLC comparison with authentic samples suggested that FGs having glucose at the 2″-position of glucose or galactose that is bound to the 3-position of kaempferol were present in Nezumisaya, whereas FGs of Harosoy were devoid of 2″-glucose. Conversely, FGs having glucose at the 6″-position of glucose or galactose that is bound to the 3-position of kaempferol were present in Harosoy, whereas these FGs were absent in Nezumisaya. Genetic analysis suggested that two genes control the pattern of attachment of these sugar moieties in FGs. One of the genes may be responsible for attachment of glucose to the 2″-position, probably encoding for a flavonol 3-<italic>O</italic>-glucoside/galactoside (1 → 2) glucosyltransferase. Nezumisaya may have a dominant whereas Harosoy may have a recessive allele of the gene. Based on SSR analysis, linkage mapping and genome database survey, we cloned a candidate gene designated as <italic>GmF3G2″Gt</italic> in the molecular linkage group C2 (chromosome 6). The open reading frame of <italic>GmF3G2″Gt</italic> is 1380 bp long encoding 459 amino acids with four amino acid substitutions among the cultivars. The <italic>GmF3G2″Gt</italic> recombinant protein converted kaempferol 3-<italic>O</italic>-glucoside to kaempferol 3-<italic>O</italic>-sophoroside. GmF3G2″Gt of Nezumisaya showed a broad activity for kaempferol/quercetin 3-<italic>O</italic>-glucoside/galactoside derivatives but it did not glucosylate kaempferol 3-<italic>O</italic>-rhamnosyl-(1 → 4)-[rhamnosyl-(1 → 6)-glucoside] and 3-<italic>O</italic>-rhamnosyl-(1 → 4)-[glucosyl-(1 → 6)-glucoside].</p></sec><sec><title>Conclusion</title><p><italic>GmF3G2″Gt</italic> encodes a flavonol 3-<italic>O</italic>-glucoside/galactoside (1 → 2) glucosyltransferase and corresponds to the <italic>Fg3</italic> gene. <italic>GmF3G2″Gt</italic> was designated as UGT79B30 by the UGT Nomenclature Committee. Based on substrate specificity of <italic>GmF3G2″Gt</italic>, 2″-glucosylation of flavonol 3-<italic>O</italic>-glycoside may be irreconcilable with 4″-glycosylation in soybean leaves.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s12870-015-0504-7) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Buttery, Br" uniqKey="Buttery B">BR Buttery</name></author><author><name sortKey="Buzzell, Ri" uniqKey="Buzzell R">RI Buzzell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Buzzell, Ri" uniqKey="Buzzell R">RI Buzzell</name></author><author><name sortKey="Buttery, Br" uniqKey="Buttery B">BR Buttery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Buzzell, Ri" uniqKey="Buzzell R">RI Buzzell</name></author><author><name 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sortKey="Hightower, Rc" uniqKey="Hightower R">RC Hightower</name></author><author><name sortKey="Meagher, Rb" uniqKey="Meagher R">RB Meagher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mackenzie, Pi" uniqKey="Mackenzie P">PI Mackenzie</name></author><author><name sortKey="Owens, Is" uniqKey="Owens I">IS Owens</name></author><author><name sortKey="Burchell, B" uniqKey="Burchell B">B Burchell</name></author><author><name sortKey="Bock, Kw" uniqKey="Bock K">KW Bock</name></author><author><name sortKey="Bairoch, A" uniqKey="Bairoch A">A Bairoch</name></author><author><name sortKey="Belanger, A" uniqKey="Belanger A">A Belanger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morita, Y" uniqKey="Morita Y">Y Morita</name></author><author><name sortKey="Hoshino, A" uniqKey="Hoshino A">A Hoshino</name></author><author><name sortKey="Kikuchi, Y" uniqKey="Kikuchi Y">Y Kikuchi</name></author><author><name sortKey="Okuhara, H" uniqKey="Okuhara H">H Okuhara</name></author><author><name sortKey="Ono, E" uniqKey="Ono E">E Ono</name></author><author><name sortKey="Tanaka, Y" uniqKey="Tanaka Y">Y Tanaka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Iwashina, T" uniqKey="Iwashina T">T Iwashina</name></author><author><name sortKey="Benitez, Er" uniqKey="Benitez E">ER Benitez</name></author><author><name sortKey="Takahashi, R" uniqKey="Takahashi R">R Takahashi</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Buzzell, Ri" uniqKey="Buzzell R">RI Buzzell</name></author><author><name sortKey="Palmer, Rg" uniqKey="Palmer R">RG Palmer</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Sayama, T" uniqKey="Sayama T">T Sayama</name></author><author><name sortKey="Ono, E" uniqKey="Ono E">E Ono</name></author><author><name sortKey="Takagi, K" uniqKey="Takagi K">K Takagi</name></author><author><name sortKey="Takada, Y" uniqKey="Takada Y">Y Takada</name></author><author><name sortKey="Horikawa, M" uniqKey="Horikawa M">M Horikawa</name></author><author><name sortKey="Nakamoto, Y" uniqKey="Nakamoto Y">Y Nakamoto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paquette, S" uniqKey="Paquette S">S Paquette</name></author><author><name sortKey="Moller, Bl" uniqKey="Moller B">BL Moller</name></author><author><name sortKey="Bak, S" uniqKey="Bak S">S Bak</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Colombie</li></country></list><tree><noCountry><name sortKey="Di, Shaokang" sort="Di, Shaokang" uniqKey="Di S" first="Shaokang" last="Di">Shaokang Di</name><name sortKey="Iwashina, Tsukasa" sort="Iwashina, Tsukasa" uniqKey="Iwashina T" first="Tsukasa" last="Iwashina">Tsukasa Iwashina</name><name sortKey="Mori, Tetsuya" sort="Mori, Tetsuya" uniqKey="Mori T" first="Tetsuya" last="Mori">Tetsuya Mori</name><name sortKey="Murai, Yoshinori" sort="Murai, Yoshinori" uniqKey="Murai Y" first="Yoshinori" last="Murai">Yoshinori Murai</name><name sortKey="Nakabayashi, Ryo" sort="Nakabayashi, Ryo" uniqKey="Nakabayashi R" first="Ryo" last="Nakabayashi">Ryo Nakabayashi</name><name sortKey="Rodriguez, Tito O" sort="Rodriguez, Tito O" uniqKey="Rodriguez T" first="Tito O" last="Rodriguez">Tito O. Rodriguez</name><name sortKey="Saito, Kazuki" sort="Saito, Kazuki" uniqKey="Saito K" first="Kazuki" last="Saito">Kazuki Saito</name><name sortKey="Sugawara, Satoko" sort="Sugawara, Satoko" uniqKey="Sugawara S" first="Satoko" last="Sugawara">Satoko Sugawara</name><name sortKey="Takahashi, Ryoji" sort="Takahashi, Ryoji" uniqKey="Takahashi R" first="Ryoji" last="Takahashi">Ryoji Takahashi</name><name sortKey="Yan, Fan" sort="Yan, Fan" uniqKey="Yan F" first="Fan" last="Yan">Fan Yan</name><name sortKey="Yonekura Sakakibara, Keiko" sort="Yonekura Sakakibara, Keiko" uniqKey="Yonekura Sakakibara K" first="Keiko" last="Yonekura-Sakakibara">Keiko Yonekura-Sakakibara</name></noCountry><country name="Colombie"><noRegion><name sortKey="Rodas, Felipe Rojas" sort="Rodas, Felipe Rojas" uniqKey="Rodas F" first="Felipe Rojas" last="Rodas">Felipe Rojas Rodas</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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